Лаборатория экобиокатализа

Лягин Илья Владимирович, 21 июля 1983 г.
к.х.н., младший научный сотрудник
Организация и ее официальный адрес:
Кафедра Химической Энзимологии, Химический факультет
Московский Государственный Университет им. М.В. Ломоносова, 119991, Москва, Ленинские горы, 1/11
Тел. +7 (495) 939-31-70, Факс: +7 (495) 939-31-70, +7 (495) 939-54-17
E-mail: lyagin@mail.ru




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Efremenko E.N., Lyagin I.V., Senko O.V., Gudkov D.A., Varfolomeyev S.D. (2008) Application of gel systems with various biocatalysts detoxifying neurotoxic agents for pollution control, water purification, and self-defense. // In book: "Sol-gel methods for materials processing" Ser. NATO Science for Peace and Security (Eds: Innocenzi P., Zub Y.L, Kessler V.G.), ISBN: 978-1-4020-8521-5, p.77-89.
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Efremenko E.N., Lyagin I.V., Senko O.V., Stepanov N.A., Spiricheva O.V., Azizov R.E. (2007) Modern trends in application of immobilized cells for environmental bioremediation.// In book: Leading-Edge Environmental Biodegradation (Ed.L.E. Pawley), Nova Science Publishers Inc., N.-Y., Ch.1, p.11-51.

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Efremenko E.N., Lyagin I.V., Plieva F.M., Galaev I.Y., Mattiasson B. (2009) Dried-reswollen immobilized biocatalysts for detoxification of organophosphorous compounds in the flow systems.// Appl. Biochem. Biotechnol., V.159, p.251-260.
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Efremenko E.N., Lyagin I.V., Votchitseva Yu.V., Gudkov D.A., Peregudov A.A., Aliev T.K., Varfolomeev S.D. (2008) The influence of length and localization of polyhistidine tag in the molecule of organophosphorus hydrolase on the biosynthesis and behavior of fusion protein.// In book: Biotechnology: sate of the art and prospects for development (Ed. G.E.Zaikov), Nova Science Publishers Inc., N.-Y., Ch.10, p.87-101.
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Efremenko E., Lyagin I., Gudkov D., Varfolomeyev S.(2007) Immobilized biocatalysts for detoxification of neurotoxic organophosphorus compounds.// Biocatalysis Biotransform., V.25 (2-4), p.359-364.
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Efremenko E., Lyagin I., Votchitseva Y., Sirotkina M., Varfolomeyev S. (2007) Polyhistidine-containing organophosphorus hydrolase with outstanding properties.// Biocatalysis Biotransform., V.25 (1), p.103-108.

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Lyagin I., Ivanov R., Lozinsky V., Efremenko E., Varfolomeyev S. (2009) Increase of stability of organophosphorus hydrolase by immobilization procedure.// XVII International Conference on Bioencapsulation, Groningen, Netherlands, September 24-26, p.146-147.
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Lyagin I., Gudkov D., Verkhusha V., Efremenko E. (2006) Genetic construct encoding the biosynthesis of N-His6-e-pHluorins-OPH in E.coli cells.// VI Annual Intern. Conference ИБХФ РАН - ВУЗы, Москва, 24-27 ноября, p.145-151.
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Spiricheva O., Senko O., Lyagin I., Lozinsky V., Varfolomeyev S., Efremenko E. (2007) Lactic acid production by immobilized fungus cells Rhizopus oryzae scaled up to the complex experimental system.// XV International workshop on Bioencapsulation., Vienna, Austria, September 6-8, P3-04, p.1-4.
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Lyagin I., Ivanov R., Lozinsky V., Efremenko E. (2007) Design of immobilized biocatalysts for degradation of neurotoxic organophosphorous compounds.// XV International workshop on Bioencapsulation. Vienna, Austria, September 6-8, P2-13, p.1-4.

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Lyagin I.V., Senko O.V., Ivanov R.V., Lozinsky V.I., Efremenko E.N., Varfolomeyev S.D. Biodestruction of neurotoxic organophosphorous pesticides in aqueous systems using heterogeneous biocatalysts.// XVII International Conference “Biocatalysis-2009: Fundamentals & Applications”, 2009, Arkhangelsk, Russia, June 19-24, p.104-105.
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Gudkov D.A., Lyagin I.V., Efremenko E.N., Verkhusha V.V. Chimeric proteins possessing organophosphorus hydrolase activity and deGFP4 protein fluorescence: synthesis in E.coli cells and features investigation.// XVII International Conference “Biocatalysis-2009: Fundamentals & Applications”, 2009, Arkhangelsk, Russia, June 19-24, p.96-97.
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Senko O.V., Lyagin I.V., Ivanov R.V., Lozinsky V.I., Efremenko E.N. Enzyme hydrolyzates of organophosphorous pesticide decomposition by neans of immobilized filamentous fungal cells. // III Int. Conference MICROBIAL DIVERSITY: current situation, conservation strategy and biotechnological potentialities, Perm-N.Novgorod-Perm, September 28 - October 05, 2008, p.199.
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Gudkov D.A., Lyagin I.V., Verkhusha V., Еfremenko Е.N. (2007) Chimeric proteins possessing features of both fluorescent protein and organophorus hydrolase. // ХVIII Mendeleev Congress on General and Applied Chemistry, Moscow, Russia, 23-28 September, p. 526.
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Lyagin I.V., Sirotkina M.S., Efremenko E.N., Varfolomeyev S.D. (2007) Enzymatic cleavage of C-P bond in organophosphorous compounds. // ХVIII Mendeleev Congress on General and Applied Chemistry, Moscow, Russia, September 23-28, p. 554.
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Lyagin I.V., Efremenko E.N., Varfolomeyev S.D. (2007) Enzymatic hydrolysis of organophosphorous compounds in flow-through system. // Intern. Conference , Moscow - St.-Petersburg, Russia, 17-22 June, p. 111-112.
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Gudkov D.A., Lyagin I.V., Verkhusha V., Еfremenko Е.N. (2007) Obtaining and investigation of properties of fusion protein containing both superecliptic-pH-sensitive fluorine and organophosphorus hudrolase. // Intern. Conference . Moscow - St.-Petersburg, Russia, 17-22 June, p. 98-99.
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Gudkov D., Lyagin I., Sirotkina M., Verkhusha V., Efremenko E. (2007) Chimeric proteins based on organophosphorus hydrolase. // 4th Moscow International Congress , Moscow, 12-16 of March, p. 279.
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Lyagin I.V., Gudkov D.A., Verkhusha V.V., Efremenko E.N. (2007) Molecular messengers for organophosphorous toxins presence in the body cells. // Intern. Workshop , Pattaya - Bangkok, Thailand, February 26 - March 8, p. 43-44.
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Efremenko E., Lyagin I., Gudkov D., Peregudov A. (2006) Immobilized biocatalysts for detoxification of neurotoxic organophosphorus compounds. // 1st Intern. Congress , Cordoba, Spain, April 23-26, p. 63.
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Gudkov D.A., Lyagin I.V., Efremenko E.N. (2005) Development of soluble and immobilized preparations of organophosphate hydrolase to solve the issues of environmental pollution with organophosphorus compounds. // VI Intern. Conf. on Environmental Pollution - ICEP 2005, Perm-Kazan-Perm, 20-25 of September, p. 149.