Hydrolases Catalytic Sites
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Kappa-carrageenase

Protein Information
NameKappa-carrageenase
SwissProtCGKA_ALTCA (P43478)
EC3.2.1.83 Kappa-carrageenase
(Hydrolases; Glycosylases; Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds; Kappa-carrageenase)
OrganismPseudoalteromonas carrageenovora
(Bacteria; Proteobacteria; Gammaproteobacteria; Pseudoalteromonadaceae; Pseudoalteromonas carrageenovora)
HCS ClassA.02.01 Glycosidases with retaining mechanism
Catalytic site residues assigned by literature reference [1] on 1DYP:A.
SCOPb.29.1.2 beta-Glucanase-like
(All beta proteins; Concanavalin A-like lectins/glucanases; beta-Glucanase-like)
CATH2.60.120.200 Jelly Rolls
(Mainly Beta; Sandwich; Jelly Rolls)
SequenceMKPISIVAFP IPAISMLLLS AVSQAASMQP PIAKPGETWI LQAKRSDEFN VKDATKWNFQ TENYGVWSWK NENATVSNGK LKLTTKRESH QRTFWDGCNQ QQVANYPLYY TSGVAKSRAT GNYGYYEARI KGASTFPGVS PAFWMYSTID RSLTKEGDVQ YSEIDVVELT QKSAVRESDH DLHNIVVKNG KPTWMRPGSF PQTNHNGYHL PFDPRNDFHT YGVNVTKDKI TWYVDGEIVG EKDNLYWHRQ MNLTLSQGLR APHTQWKCNQ FYPSANKSAE GFPTSMEVDY VRTWVKVGNN NSAPGEGQSC PNTFVAVNSV QLSAAKQTLR KGQSTTLEST VLPNCATNKK VIYSSSNKNV ATVNSAGVVK AKNKGTATIT VKTKNKGKID KLTIAVN

Residues of active site
Residue nameResidueRole
NGlu163Nucleophile
AGlu168General acid

Reference
  1. Michel G, Chantalat L, Duee E, Barbeyron T, Henrissat B, Kloareg B, Dideberg O
    The kappa-carrageenase of P. carrageenovora features a tunnel-shaped active site: a novel insight in the evolution of Clan-B glycoside hydrolases.
    Structure. (Camb). (2001). 9 (6), 513-25. pubmed: 11435116

PDB Structure Information
PDB codeTitle
1DYP1,3-alpha-1,4-beta-d-galactose-4-sulfate- 3,6-anhydro-d-galactose 4 galactohydrolase
ChainResiduesNotes
AGlu163, Glu168 Residues from 27 to 297.


Hydrolases Catalytic Sites
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